Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells
ਵੈਬAdd 2 vol DPSC culture medium, transfer the cells to a 15-ml centrifuge tube, and centrifuge the tubes 5 min at 300 × g, room temperature, to pellet the cells. Aspirate the supernatant and resuspend the pellet in freezing medium at a density of 5 × 10 5 cells/ml. Transfer the resuspended cells to cryotubes.
Cytogenetic instability of dental pulp stem cell lines - Springer
ਵੈਬCytogenetic instability of dental pulp stem cell lines ... cell lines that were analysed using G banding (Tables 1, 2). The FISH approach is an alternative to the analysis when the cell pellet that was obtained after fixation was reduced or insufficient for a G-banding conventional analysis. As a control, normal human lymphocytes were analyzed in
Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping ...
ਵੈਬ9 ਜਨ 2023 · This involves the preliminary assessment of unspecific toxicity in cell lines, the growth inhibition of microorganisms, and the evaluation of genotoxicity, mutagenicity, and carcinogenicity in bacteria . ... In vitro cytotoxicity and functional differentiation experiments with human dental pulp cells are effective for analyzing cell reactivity, behavior, and …
Dental pulp stem cells in regenerative medicine - Nature
ਵੈਬDental pulp stem cells in regenerative medicine P. Hollands,*1 D. Aboyeji 1 and M. Orcharton1 form dentine, pulp, cementum and the peri-odontal ligament. 1 Five subtypes of mesenchy - mal stem ...
1.10: Dentin-Pulp Complex Development - Medicine LibreTexts
ਵੈਬOdontoblasts (arrows in figure 228) are the cells that produce dentin, first by secreting proteins including collagen that act as a scaffold. Mineralization around the scaffold occurs later. The initial protein-rich material is pre-dentin (P in Fig. 10.2), after it mineralizes it is called dentin (D in Fig. 10.2).
9. Dental pulp | Pocket Dentistry
ਵੈਬ4 ਜਨ 2015 · Undifferentiated cells. Overview. Dental pulp is the soft, loose connective tissue located in the central portion of each tooth. It has a crown (coronal part) and a root (radicular part). Pulp is a delicate, specialized connective tissue containing thin-walled blood vessels, nerves, and nerve endings enclosed within dentin. ... Odontoblasts line the …
Dental cell type atlas reveals stem and differentiated cell types in ...
ਵੈਬ23 ਸਤੰ 2020 · e Landscape of mesenchymal cells of dental pulp (dots) and follicle (crosses) is reproduced and extended with 10× Chromium. t-SNE embedding shows 2552 mesenchymal cells grouped in clusters, where ...
Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem ...
ਵੈਬT32 DE018381/DE/NIDCR NIH HHS/United States. This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequen ….
5: Dentin, Pulp, and Tooth Pain | Pocket Dentistry
ਵੈਬ4 ਜਨ 2015 · The odontoblast and Hoehl’s cell layers include endothelial cells forming capillaries (cp). The dental pulp is located more centrally. Bar = 10 μm. (b) Hematoxylin- and eosin-stained section of a human molar. Labels as in 5.3(a). Arrowheads indicate odontoblast processes, and arrows indicate Hoehl’s cells. Evolution of dental structures
Dental Pulp Stem Cells: Advances to Applications - PubMed
ਵੈਬ13 ਫ਼ਰ 2020 · Dental pulp stem cells (DPSCs) have a high capacity for differentiation and the ability to regenerate a dentin/pulp-like complex. Numerous studies have provided evidence of DPSCs' differentiation capacity, such as in neurogenesis, adipogenesis, osteogenesis, chondrogenesis, angiogenesis, and dentinogenesis. The molecular …
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