Dental cell type atlas reveals stem and differentiated cell types in ...
WEBSep 23, 2020 · e Landscape of mesenchymal cells of dental pulp (dots) and follicle (crosses) is reproduced and extended with 10× Chromium. t-SNE embedding shows 2552 mesenchymal cells grouped in clusters, where ...
Immortalized cell lines derived from dental/odontogenic tissue
WEBApr 11, 2023 · An immortalized mouse floxed Bmp2 dental papilla mesenchymal cell line (iBmp2-dp cells) was generated for studying the mechanisms of stem cells from dental/odontogenic tissue differentiation and dentin formation mediated by Bmp2 (Wu et al. 2010 ). Furthermore, Cre fluorescent protein (GFP) was inducted into iBmp2-dp cells to …
Establishment of Stable Cell Lines from Primary Human Dental Pulp …
WEBThis protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars, and for the generation of high titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.
Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental …
WEBAim: To investigate the cytotoxicity of five root canal sealers on L929 mouse fibroblasts and primary human dental pulp cells. Methodology: Cylindrical specimens of AH Plus (Dentsply De Trey GmbH, Konstanz, Germany), RoekoSeal (Coltène Whaledent, Langenau, Germany), EndoREZ (Ultradent Products Inc., South Jordan, UT, USA), …
Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells
WEBAdd 2 vol DPSC culture medium, transfer the cells to a 15-ml centrifuge tube, and centrifuge the tubes 5 min at 300 × g, room temperature, to pellet the cells. Aspirate the supernatant and resuspend the pellet in freezing medium at a density of 5 × 10 5 cells/ml. Transfer the resuspended cells to cryotubes.
Effects of Hyaluronic Acid Sponge as a Scaffold on Odontoblastic Cell …
WEBJan 1, 2010 · Based on the abundant CDots-induced ECM and thereby the reinforcement of the cell-ECM adhesion, an intact dental pulp stem cell sheet can be achieved, which in return promote in vivo the efficient ...
Effects of hyaluronic acid sponge as a scaffold on odontoblastic cell ...
WEBIn the present study, the effects of hyaluronic acid (HA) sponge on responses of the odontoblastic cell line (KN-3 cells) in vitro, as well as responses of amputated dental pulp of rat molar in vivo, were examined. In vitro, KN-3 cells adhered to the stable structure of HA sponge and that of collagen sponge. In vivo, dental pulp proliferation ...
The roles of odontoblasts in dental pulp innate immunity
WEBMar 27, 2018 · In contrast, human dental pulp cells (HDPCs) showed a low level of NOD2 protein expression . Another study reported that significant up-regulation of NOD2 gene as well as tumor necrosis factor (TNF)-α occurs in acute inflamed human dental pulps compared to healthy ones. ... IL-8, and TNF-α in a rat odontoblast-like cell line via the …
Assessing the effect of human dental pulp mesenchymal stem cell ...
WEBBackground: The secretome of the dental pulp mesenchymal stem cells (DPMSCS-S) have an array of regenerative potential and could aid in the rehabilitation of cancer patients post-therapeutic interventions, although caution is required as DPMSC-S have shown to augment prostate cancer cells. Thus, it is vital to assess if these pro-carcinogenic …
In vitro cytotoxicity of different dental resin-cements on human cell lines
WEBJan 20, 2021 · A few number of recent in vitro studies are focused on the cellular and cytotoxic effects of various composite cements on oral fibroblasts such as dental pulp cells or permanent tumor cell lines [21, 22, 26]. In implant dentistry, peri-implant diseases are arising.
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