Establishment of Stable Cell Lines from Primary Human Dental Pulp …
ওয়েবStable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments. Keywords: Lentivirus, Gene Transfer Techniques, Genetic Transduction, Genetic Recombination, Somatic Stem Cells. 1. Introduction. Lentiviral expression vector systems have been used extensively to overexpress proteins in …
Characterization of human dental pulp cells grown in …
ওয়েবThe dental pulp cell lines (A) DPCs-2, (B) DPCs-3, (C) DPCs-4 and (D) DPCs-5 were seeded at low density (5×103 cells/cm2) and pre-cultured for 7 days in DMEM/10% FBS or STK2. When cultures reached …
Effects of Er:YAG and Diode Laser Irradiation on …
ওয়েব17 আগস্ট, 2018 · In dental research, dental pulp tissues containing DPSCs have been used to establish a number of odontoblastic cell lines from rat [21,22], mouse , cow , pig , and human [26,27]. Pulp capping …
Pulp Anatomy and Characterization of Pulp Cells | SpringerLink
ওয়েব1 জানু, 2014 · The A4 lines appeared to be multipotent cells. In contrast C5 and H8 displayed a more restricted potential and were monopotent. In the dental pulp, progenitor cells demonstrate the coexistence of multipotent cells and cells with restricted lineages . The A4 cells express DMP-1 after 4 days of culture, reaching a maximum at 7 days. …
Establishment of porcine pulp-derived cell lines and ... - PubMed
ওয়েবWe established cell lines from developing porcine dental papillae and used them to express recombinant dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP1). Pulp cells were immortalized with pSV3-neo and clonally selected. Cell lines were characterized by reverse transcruption-polymerase chain reaction (RT-PCR) and …
Dental pulp cells cocultured with macrophages aggravate the ...
ওয়েব9 ডিসেম্বর, 2023 · Background Pulp inflammation is complex interactions between different types of cells and cytokines. To mimic the interactions of different types of cells in inflamed dental pulp tissues, dental pulp cells (DPCs) were cocultured with different ratios of macrophages (THP-1) or LPS treatment. Methods DPCs were cocultured with …
Determinants of Dental Pulp Stem Cell Heterogeneity
ওয়েব6 জুলাই, 2022 · In this study, we established a novel immortalized dental pulp stem cell line by co-expressing a mutant cyclin-dependent kinase 4 (CDK4R24C), Cyclin D1, and telomerase reverse transcriptase (TERT ...
From Pluripotent Stem Cells to Organoids and Bioprinting
ওয়েব18 মার্চ, 2024 · Reciprocal epithelial-mesenchymal interactions are the driving forces throughout the various stages of tooth development. Whereas the dental mesenchymal compartment contributes to most cells in mature tooth, including dental pulp and periodontal ligament, as well as to the dentin layer which is produced by odontoblasts, …
Therapeutic Potential of Dental Pulp Stem Cells According to
ওয়েব7 ডিসেম্বর, 2021 · Stem cells are unspecialised cells capable of perpetual self-renewal, proliferation and differentiation into more specialised daughter cells. They are present in many tissues and organs, including the stomatognathic system. Recently, the great interest of scientists in obtaining stem cells from human teeth is due to their easy …
The roles of odontoblasts in dental pulp innate immunity
ওয়েব27 মার্চ, 2018 · In contrast, human dental pulp cells (HDPCs) showed a low level of NOD2 protein expression . Another study reported that significant up-regulation of NOD2 gene as well as tumor necrosis factor (TNF)-α occurs in acute inflamed human dental pulps compared to healthy ones. ... IL-8, and TNF-α in a rat odontoblast-like cell line …
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