Dental Pulp Stem Cells: Advances to Applications - PubMed
ਵੈਬ13 ਫ਼ਰ 2020 · Dental pulp stem cells (DPSCs) have a high capacity for differentiation and the ability to regenerate a dentin/pulp-like complex. Numerous studies have provided evidence of DPSCs' differentiation capacity, such as in neurogenesis, adipogenesis, osteogenesis, chondrogenesis, angiogenesis, and dentinogenesis. The molecular …
Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells
ਵੈਬAdd 2 vol DPSC culture medium, transfer the cells to a 15-ml centrifuge tube, and centrifuge the tubes 5 min at 300 × g, room temperature, to pellet the cells. Aspirate the supernatant and resuspend the pellet in freezing medium at a density of 5 × 10 5 cells/ml. Transfer the resuspended cells to cryotubes.
Single-cell characterization of monolayer cultured human dental pulp ...
ਵੈਬ15 ਦਸੰ 2021 · Cellular composition in human dental pulps. To explore the cellular composition of human dental pulp tissues, we first used scRNA-seq to profile 7000 isolated cells from the fresh erythrocyte ...
Full-length amelogenin influences the differentiation of human dental …
ਵੈਬ13 ਜਨ 2016 · Amelogenin is an extracellular matrix protein well known for its role in the organization and mineralization of enamel. Clinically, it is used for periodontal regeneration and, due to its finding also in predentin and intercellular spaces of dental pulp cells, it has recently been suggested for pulp capping procedures. The aim of this study was to …
In vitro cytotoxicity of different dental resin-cements on human cell lines
ਵੈਬ20 ਜਨ 2021 · A few number of recent in vitro studies are focused on the cellular and cytotoxic effects of various composite cements on oral fibroblasts such as dental pulp cells or permanent tumor cell lines [21, 22, 26]. In implant dentistry, peri-implant diseases are arising.
From Pluripotent Stem Cells to Organoids and Bioprinting
ਵੈਬ18 ਮਾਰਚ 2024 · Reciprocal epithelial-mesenchymal interactions are the driving forces throughout the various stages of tooth development. Whereas the dental mesenchymal compartment contributes to most cells in mature tooth, including dental pulp and periodontal ligament, as well as to the dentin layer which is produced by odontoblasts, …
Cells | Free Full-Text | Mechanobiology of Dental Pulp Cells - MDPI
ਵੈਬ21 ਫ਼ਰ 2024 · The dental pulp is the inner part of the tooth responsible for properly functioning during its lifespan. Apart from the very big biological heterogeneity of dental cells, tooth microenvironments differ a lot in the context of mechanical properties—ranging from 5.5 kPa for dental pulp to around 100 GPa for dentin and enamel. This physical …
A Study on the Effect of Human Dental Pulp Stem Cell
ਵੈਬ31 ਜੁਲਾ 2019 · Immortalized cell lines can be established through several methods, ... Dental pulp stem cells (DPSCs) are having a MSCs phenotype and they are differentiated into neuron, cardiomyocytes ...
Immortalization of human dental papilla, dental pulp ... - PubMed
ਵੈਬThese cell lines will be useful tools for studying the repair and regeneration of dental and periodontal tissues and various diseases including odontogenic tumors. Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase J Oral Pathol Med. 2004 Aug;33(7):417-23. doi: …
Characterization of human dental pulp cells grown in chemically …
ਵੈਬThe dental pulp cell lines (A) DPCs-2, (B) DPCs-3, (C) DPCs-4 and (D) DPCs-5 were seeded at low density (5×103 cells/cm2) and pre-cultured for 7 days in DMEM/10% FBS or STK2. When cultures reached confluence, the cells were cultured in osteogenesis induction medium for up to 16 days. ALP activity was determined on the indicated days following …
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