Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells
WEBAdd 2 vol DPSC culture medium, transfer the cells to a 15-ml centrifuge tube, and centrifuge the tubes 5 min at 300 × g, room temperature, to pellet the cells. Aspirate the supernatant and resuspend the pellet in freezing medium at a density of 5 × 10 5 cells/ml. Transfer the resuspended cells to cryotubes.
Effects of Hyaluronic Acid Sponge as a Scaffold on Odontoblastic Cell …
WEBJan 1, 2010 · Based on the abundant CDots-induced ECM and thereby the reinforcement of the cell-ECM adhesion, an intact dental pulp stem cell sheet can be achieved, which in return promote in vivo the efficient ...
Assessing the effect of human dental pulp mesenchymal stem cell ...
WEBBackground: The secretome of the dental pulp mesenchymal stem cells (DPMSCS-S) have an array of regenerative potential and could aid in the rehabilitation of cancer patients post-therapeutic interventions, although caution is required as DPMSC-S have shown to augment prostate cancer cells. Thus, it is vital to assess if these pro-carcinogenic …
In vitro cytotoxicity of different dental resin-cements on human cell lines
WEBJan 20, 2021 · A few number of recent in vitro studies are focused on the cellular and cytotoxic effects of various composite cements on oral fibroblasts such as dental pulp cells or permanent tumor cell lines [21, 22, 26]. In implant dentistry, peri-implant diseases are arising.
Dental Pulp Stem Cells: What’s New? | SpringerLink
WEBMay 25, 2017 · The schematic represents a summary of the individual (solid lines) or multifaceted (dashed lines) approaches employed to regenerate (a) the pulp tissue or (b) tooth like structure using DPSC from various animals in proof-of-principle studies.(a) Dental pulp regeneration has encompassed the use of numerous biomaterials to generate an …
Effects of hyaluronic acid sponge as a scaffold on odontoblastic cell ...
WEBIn the present study, the effects of hyaluronic acid (HA) sponge on responses of the odontoblastic cell line (KN-3 cells) in vitro, as well as responses of amputated dental pulp of rat molar in vivo, were examined. In vitro, KN-3 cells adhered to the stable structure of HA sponge and that of collagen sponge. In vivo, dental pulp proliferation ...
Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental …
WEBAim: To investigate the cytotoxicity of five root canal sealers on L929 mouse fibroblasts and primary human dental pulp cells. Methodology: Cylindrical specimens of AH Plus (Dentsply De Trey GmbH, Konstanz, Germany), RoekoSeal (Coltène Whaledent, Langenau, Germany), EndoREZ (Ultradent Products Inc., South Jordan, UT, USA), …
Full-length amelogenin influences the differentiation of human dental …
WEBJan 13, 2016 · Amelogenin is an extracellular matrix protein well known for its role in the organization and mineralization of enamel. Clinically, it is used for periodontal regeneration and, due to its finding also in predentin and intercellular spaces of dental pulp cells, it has recently been suggested for pulp capping procedures. The aim of this study was to …
Human dental pulp stem cells: Applications in future regenerative ...
WEBJun 6, 2015 · INTRODUCTION. Stem cells are unspecialized cells having a property of self renewal and further differentiate into various types of specialized cells[].Stem Cells are identified in a number of adult tissues including skin, adipose tissues[], peripheral blood[3,4], bone marrow, pancreas, intestine, brain, hair follicles, as well as in the dental pulp …
Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem ...
WEBT32 DE018381/DE/NIDCR NIH HHS/United States. This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequen ….