Isolation and culture of dental pulp stem cells from permanent …
వెబ్We report a protocol for the extirpation of dental pulp tissue, in-vitro explant culture and propagation of dental pulp MSCs from permanent and deciduous human teeth. MSCs are comprised of heterogeneous population thus it is important to characterize them for the presence of stem cell sub-populations.
Isolation and Culture of Primary Human Dental Pulp Cells—A …
వెబ్1, మార్చి 2024, · The primary aim of this methodological study is to provide a detailed description and visualisation of the methods required to extract dental pulp from the pulp chamber for culture using an explant outgrowth method.
Isolation and Culture of Primary Human Dental Pulp Cells-A …
వెబ్1, మార్చి 2024, · Here, we describe a modified method for the efficient isolation of primary human DPCs from sound third molar teeth for culture using an explant outgrowth method on basement membrane-coated flasks, as well as using high-resolution macro-photography to illustrate the methods.
(PDF) Isolation and Culture of Primary Human Dental Pulp …
వెబ్1, మార్చి 2024, · PDF | The dental pulp has critical functions in tooth development as well as an ongoing role in promoting and maintaining the vitality of teeth. In... | Find, read and cite all the research you ...
Dental Pulp Stem Cells: Advances to Applications - PMC
వెబ్13, ఫిబ్ర 2020, · Dental pulp stem cells (DPSCs) have a high capacity for differentiation and the ability to regenerate a dentin/pulp-like complex. Numerous studies have provided evidence of DPSCs’ differentiation capacity, such as in neurogenesis, adipogenesis, osteogenesis, chondrogenesis, angiogenesis, and dentinogenesis.
Primary Culture of Dental Pulp Stem Cells - PubMed
వెబ్5, మే 2023, · There are various practical ways of harvesting, maintaining, and proliferating adult stem cells being investigated for their use in regenerative medicine. In this work, we demonstrate the establishment of a primary mesenchymal stem cell culture from dental tissue by the explant culture method.
Single-cell characterization of monolayer cultured human dental pulp ...
వెబ్15, డిసెం 2021, · International Journal of Oral Science - Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity
A Cell Culture Method for the Isolation and Study of Primary
వెబ్24, నవం 2022, · Primary human dental pulp cell (HDPC) cultures contain dental pulp stem cell (DPSC) populations. DPSCs are multipotent mesenchymal cells residing inside the dental pulp where they can contribute to the regenerative potential of this and other tissues throughout the body.
Isolation, Culture, and Characterization of Dental Pulp Stem Cells …
వెబ్17, మే 2024, · The protocol outlines the isolation, culture, and characterization of dental pulp stem cells (DPSCs) from human deciduous and permanent teeth. It includes a description of the storage and proliferation of these cells, as well as the assessment of their in vitro differentiation potential into osteoblasts, adipocytes, and chondrocytes 35 .
Dental Pulp Stem Cell (DPSC) Isolation, Characterization, and ...
వెబ్Dental pulp stem cells (DPSC) have been proposed as an alternative to pluripotent stem cells to study multilineage differentiation in vitro and for therapeutic application. Standard culture media for isolation and expansion of stem cells includes.
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